N/A

MKHHHHHHAS PGAGRRGSRP GAVGGAVDAD VGEEGVQQAL DFAVREYNRA SNDAYHSRAL RVLRARKQVV SGMNYFLEVE IGRTRCTKSQ PNLDNCPFHD QPHLMRKTLC SFQIYTVPWL GKTSLVKSSC QDV

Western blotting

K9 Cystatin-Cwas filtered (0.4um) and lyophilized from 0.5mg/ml supplied in 20mM TRIS, 50mM NaCl, pH 7.5.

BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.

Add deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

E. coli

Store lyophilized Cystatin-C K9 at -20^oC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Cystatin-C can be stored at 4^oC for a limited period of time; it does not show any change after two weeks at 4^oC.

Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.