Cytotoxic T-Lymphocyte Associated Antigen-4/ Fc Chimera Human Recombinant
Cytotoxic T-Lymphocyte Associated Antigen-4 Human Recombinant produced in insect cells is a homodimeric, glycosylated, polypeptide chain containing a total molecular mass of 80 kDa. Each subunit (40kDa) is fused to a polypeptide linker to the Fc portion of Human IgG1. CTLA4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo.
Large doses of this soluble protein also suppress responses to a second immunization. CTLA-4 is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
The sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val.
Formulation
CTLA-4 was lyophilized with no additives.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Cytotoxic T-Lymphocyte Associated Antigen-4 Human in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized Cytotoxic T-Lymphocyte Associated Antigen Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution CTLA-4 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
GSE, CD152, IDDM12, CELIAC3, CTLA-4.
