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MCSF Rat Recombinant produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254). M-CSF is disulfide-linked homodimer containing 2 x 222 a.a chains.
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The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.
The recombinant MCSF was lyophilized after extensive dialysis against PBS.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
It is recommended to reconstitute the MCSF in sterile PBS not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
HEK293
Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution MCSF should be stored at 4oC between 2-7 days and for future use below -18oC.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.
