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Recombinant Cruciform DNA-recognizing protein 1 (CRP1)

Recombinant Cruciform DNA-recognizing protein 1 (CRP1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520684SVOYeast1mgQuote
EP520684SVOE. coli1mgQuote
BP520684SVOBaculovirus200ugQuote
MP520684SVOMammalian Cell200ugQuote

Protein Information

SpeciesSaccharomyces cerevisiae (strain Lalvin QA23) (Baker's yeast)
UniProt IDE7KPJ0
Gene NameCRP1; ORFs:QA23_2200
Protein NameCruciform DNA-recognizing protein 1 Cleaved into the following 2 chains: 1. CRP1 short N-terminal subpeptide 2. CRP1 short C-terminal subpeptide
Region Expressed161-465
Expression Tag6xHis
Purity>90%
AA SequenceEEEDGVTGTTTEDVTGTSREETPLAEPTNVSKEAPGNFHILPIDQSADTTKSNGIIGGPG PVLVPNPGEIKEFTEIRDVDARELNERLNKKEEVPEPVAGPIVESSVTEKSPALPQADDP IVETKEVAHNVQELTPQVEAVTPLINEPEPLPTPEAQISIPESSKVEPVEGSLQSKLVEK RESTEGVLDGSKKVENKAKKDEEVFTLDPIVNKAPKLPLTDEQTAEGRKSPAVSEEKEKK KKQEKGSKEVKRSETSKEKKPSAKEVKKQTVKASKKQTASPLSSSTEEPKKKKTGFFGKL KKLFK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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