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Recombinant Choline dehydrogenase (betA)

Recombinant Choline dehydrogenase (betA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP005335Yeast1mgQuote
EP005335E. coli1mgQuote
BP005335Baculovirus200ugQuote
MP005335Mammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 17760 / NCIB 9086 / R18194) )
UniProt IDQ39A44
Gene NamebetA; Locus:Bcep18194_B0553
Protein NameCholine dehydrogenase
Region Expressed1-566
Expression Tag6xHis
Purity>90%
AA SequenceMTTREYDYIICGAGSAGNVLATRLTEDPDVTVLLLEAGGPDYRFDFRTQMPAALAYPLQG RRYNWAYETDPEPHMDNRRMECGRGKGLGGSSLINGMCYIRGNALDYDNWSTHKGLENWT YLDCLPYFKKAETRDVGPNDYHGGNGPVSVTTSKPGVNPLFEAMVDAGVQAGYPRTDDLN GYQQEGFGPMDRTVTPKGRRASTARGYLDQAKVRPNLEIVTHALADRILFDGKRASGVTY LRGSERATAHARREVLVCSGAIASPQLLQRSGVGPGAWLKELDIPVVLDLPGVGQNLQDH LEMYIQYECKEPVSLYPALKWWNQPKIGLEWMLNGTGLGASNHFEAGGFIRTRDDDLWPN IQYHFLPVAINYNGSNAIEMHGFQAHVGSMRSPSRGRVKLRSRDPNDHPSILFNYMAEAL DWREFRDAIRATREIMRQPALDRYRGRELNPGADCKSDKELDAFVRARAETAFHPSCSCK MGYDDMAVVDEEGRVHGLEGLRVVDASIMPIITTGNLNAPTIMIAEKIADKIRGRKALAR VDVPYFVANGAMARNIAKAVRQPETV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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