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Recombinant Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1)

Recombinant Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP006389GOYeast1mgQuote
EP006389GOE. coli1mgQuote
BP006389GOBaculovirus200ugQuote
MP006389GOMammalian Cell200ugQuote

Protein Information

SpeciesCapra hircus (Goat)
UniProt IDP79153
Gene NameCYP11A1
Protein NameCholesterol side-chain cleavage enzyme, mitochondrial
Region Expressed40-520
Expression Tag6xHis
Purity>90%
AA SequenceISTRTPRPYSEIPSPGDNGWINLYHFWRKKSSQRIHFRHIENFQKYGPIYREKLGNLESV YIIHPEDVAHLFKFEGSYPQRYDIPPWLAYHQYYQKPIGVLFKKSGAWKKDRVVLNTEVM APEAIKNFIPLLNPVSQDFVSLLRKRIQQQGSGKFAGDIKEDLFHFAFESITNVMFGERL GMLEDTVNTEAQKFIDAVYKMFHTSVPLLNLPPELYRLFRTKTWRDHVAAWDTIFNKAEK YTEIFYQDLRQKTEFRNYPGILYHLLKSEKMLLEDVKANITEMLAGGVDTTSMTLQWHLY EMARSLNVQEMLREEVLNARRQAEGDISKMLQMVPLLKASIKETLRLHPISVTLQRYPES DLVLQDYLIPAKTLVQVAIYAMGRDPAFFSNPDKFDPTRWLGKDKDLIHFRNLGFGWGVR QCVGRRIAELEMTLFLIHILENFKIEMQQIGDVNTIFNLILTPDKPIFLVFRPFNQDPPQ A
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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