Request QuoteCatalog Number: xP006460DXUSize: 0.2-1mg

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Recombinant Cholesterol 7-alpha-monooxygenase (CYP7A1)

Recombinant Cholesterol 7-alpha-monooxygenase (CYP7A1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP006460DXUYeast1mgQuote
EP006460DXUE. coli1mgQuote
BP006460DXUBaculovirus200ugQuote
MP006460DXUMammalian Cell200ugQuote

Protein Information

SpeciesCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
UniProt IDP46634
Gene NameCYP7A1; aka: CYP7
Protein NameCholesterol 7-alpha-monooxygenase
Region Expressed1-504
Expression Tag6xHis
Purity>90%
AA SequenceMMTISLIWGIAMVVCCCIWVIFDRRRRKAGEPPLENGLIPYLGCALKFGSNPLEFLRANQ RKHGHVFTCKLMGKYVHFITNSLSYHKVLCHGKYFDWKKFHYTTSAKAFGHRSIDPNDGN TTENINNTFTKTLQGDALHSLSEAMMQNLQFVLRPPDLPKSKSDAWVTEGMYAFCYRVMF EAGYLTLFGRDTSKPDTQRVLILNNLNSFKQFDQVFPALVAGLPIHLFKAAHKAREQLAE GLKHENLSVRDQVSELIRLRMFLNDTLSTFDDMEKAKTHLAILWASQANTIPATFWSLFQ MIRSPDALRAASEEVNGALQSAGQKLSSEGNAIYLDQIQLNNLPVLDSIIKEALRLSSAS LNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPAIYPDPLTFKYDRYLDENKKAK TSFYSNGNKLKYFYMPFGSGATICPGRLFAVQEIKQFLILMLSYFELELVESHVKCPPLD QSRAGLGILPPLNDIEFKYKLKHL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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