Western blot withmyositis sera ormonoclonal anti-hexa-His-tag antibody.
0.3-0.7 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
CHD4 is supplied in 20mM HEPES buffer pH-8.0 and 500mM NaCl.
Binds IgG-type human auto-antibodies.
Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Greater than 80% as determined by SDS-PAGE.
Store at 4oC if entire vial will be used within 2-4 weeks. Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Chromodomain Helicase DNA binding protein 4, Mi-2b, Mi2-BETA, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, EC 3.6.4.12, EC 3.6.1.