Centromere Protein B Human Recombinant
CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 Dalton.
CENPB is expressed with a -6x His tag and purified by proprietary chromatographic techniques.
Accession
Applications
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating concentration
0.15-0.375 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Formulation
CENPB is supplied in 20mM HEPES buffer pH-7.6, 250mM sodium chloride, and 20% glycerol.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Physical Appearance
Sterile Filtered liquid formulation.
Purity
Greater than 90% as determined by SDS-PAGE.
Source
Storage Conditions
Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.
