$148.20Catalog Number: PTN390-20ugSize: 20ug

CENPB Human

Centromere Protein B Human Recombinant

CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 Dalton.
CENPB is expressed with a -6x His tag and purified by proprietary chromatographic techniques.

Accession

P07199

Applications

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Coating concentration

0.15-0.375 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Formulation

CENPB is supplied in 20mM HEPES buffer pH-7.6, 250mM sodium chloride, and 20% glycerol.

Immunological functions

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Physical Appearance

Sterile Filtered liquid formulation.

Purity

Greater than 90% as determined by SDS-PAGE.

Source

Sf9 insect cells.

Storage Conditions

Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.

Synonyms

Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.

Quantity

$148.20

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