CD45 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.

P3G4AT.

Mouse IgG2a heavy chain and κ light chain.

Anti-human CD45 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CD45 amino acids 1029-1249 purified from E. coli.

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

CD45 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

12 months at -20^oC. 1 month at 4^oC.

For periods up to 1 month store at 4^oC, for longer periods of time, store at -20^oC. Prevent freeze thaw cycles.

Leukocyte common antigen, EC 3.1.3.48, L-CA, T200, CD45 antigen, PTPRC, LCA, LY5, B220, CD45, GP180.

Mouse Anti Human Monoclonal.