$307.80Catalog Number: PTN537-5ugSize: 5ug

CD14 Mouse

CD14 Mouse Recombinant

The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controled by SDS page.
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.

Accession

P10810

Applications

ELISA.
Inhibition Assays.
Western Blotting.

Characterization

On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 50 kDa.

Formulation

CD14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility

It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

CHO-cells.

Stability

Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Mouse CD14 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.

Quantity

$307.80

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