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Recombinant 6-carboxyhexanoate--CoA ligase (bioW)

Recombinant 6-carboxyhexanoate--CoA ligase (bioW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518910BRMYeast1mgQuote
EP518910BRME. coli1mgQuote
BP518910BRMBaculovirus200ugQuote
MP518910BRMMammalian Cell200ugQuote

Protein Information

SpeciesBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
UniProt IDE0TXE1
Gene NamebioW; Locus:BSUW23_14650
Protein Name6-carboxyhexanoate--CoA ligase
Region Expressed1-258
Expression Tag6xHis
Purity>90%
AA SequenceMQEETFYSVRMRASMNGSHEDGGKHISGGERLIPFHEMKRTVNSLLEKGLSHSRGKPDFM QIQFEEVHEPIKTIQPLPVRTNEVSSPEEGQKLARLLLEREGVSREVIEKAYQQIISWSG VRGAVLFDIHSGKRIDQTKGKGVRVSRMDWPDANFEKWALRYHVPAHSRIKEALALASKV SRFPAAVAELCWSDDPDYITGYVAGKKMGYQRITALKEYGSEDGCRIFFIDGSEDVNTYI HDLEKQPILIEWEEDHDS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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