$90.00Catalog Number: PTN400-1ugSize: 1ug

CALB1 Rat

Calbindin-1 Rat Recombinant

Recombinant Rat Calbindin-1 produced in E.Coli.
The Rat CALB1 is purified by proprietary chromatographic techniques.

Accession

P07171

Applications

CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1 ul of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).
B- Add 1 ug of the recombinant protein to 1 ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.

Formulation

The protein was lyophilized from a concentrated solution (1mg/ml) containing 50mM NaHCO3.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 90% as determined by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized CABP28K in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution CABP28K should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326.

Quantity

$90.00

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