$330.60Catalog Number: MAB049-1mgSize: 1mg

BrdU Antibody

BrdU (5-bromo-2-deoxyuridine), Mouse Antibody
Monoclonal Antibody

Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Applications

Immunohistochemistry, flow cytometry.

Clone

YR-BrdU.

Ig Subclass

mouse IgG1

Immunogen

5-iodouridine covalently coupled to KLH.

Note

This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat.

Protein formulation

1mg/ml in PBS (after reconstitution).

Purification Method

Protein A column

Reconstitution

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Shipping Conditions

Antibody is shipped lyophilized at ambient temperature.

Storage Procedures

In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.

Titer

1:500- 1:2,000 (assay dependent)

Type

Mouse Antibody Monoclonal.

Quantity

$330.60

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