$148.20Catalog Number: PCM001-20ugSize: 20ug

BRAK Human

BRAK (CXCL14) Human Recombinant

CXCL14 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9.4kDa.
The CXCL14 is purified by proprietary chromatographic techniques.

Accession

O95715

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys.

Biological Activity

The ED50 of CXCL14 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.

Formulation

CXCL14 was lyophilized after extensive dialysis against 20mM Tris-HCl, pH 8.5 and 1M NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized CXCL14 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized CXCL14 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution CXCL14 should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.

Synonyms

C-X-C motif chemokine 14, Small-inducible cytokine B14, Chemokine BRAK, Bolekine, NJAC, KS1, Kec, BMAC, MIP-2g, SCYB14, CXCL14, BRAK, MGC10687. 

Quantity

$148.20

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