QNLDSMLHGT GMKSDSDQKK SENGVTLAPE DTLPFLKCYC SGHCPDDAIN NTCITNGHCF AIIEEDDQGE TTLASGCMKY EGSDFQCKDS PKAQLRRTIE CCRTNLCNQY LQPTLPPVVI GPFFDGSIRE NLYFQGGSGT KLDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH GDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK.
The biological activity of human BMPR1A is determined by its ability to inhibit human BMP-4 induction of alkaline phosphatase in the mouse chondrogenic cell line ATDC5. The observed ED50 range is from 0.1 to 0.3 ug/ml in the presence of 30ng/ml of recombinant human BMP-4 protein.
The BMPR1A protein was lyophilized from a 0.2um filtered solution containing no additives or preservatives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
It is recommended to reconstitute the lyophilized BMPR1A in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized BMPR1A although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BMPR1A should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
BMPR-1A, BMP-R1A, BMPR1A, BMR1A, CD292, CD-292, Serine/threonine-protein kinase receptor R5, SKR5, Activin receptor-like kinase 3, ALK-3, ACVRLK3, EC 2.7.11.30, CD292 antigen.
