$123.50Catalog Number: PYT081-10ug

BMP 4 Human, Active

Active Bone Morphogenetic Protein-4 Human Recombinant

Active human BMP-4 produced in HEK cells is a glycosylated disulfide linked homodimer, having a total molecular weight of 34kDa.
The BMP-4 is purified by proprietary chromatographic techniques.

Accession

P12644

Amino acid sequence

SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR.

Applications

The specific activity was determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) and is typically 4.53ng/ml corresponding to a Specific Activity of 220,750.55IU/mg.

Formulation

lyophilized from 0.7mg/ml in 2xPBS+6% ethanol

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-4 in sterile 100mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Bone Morphogenetic Protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BMP4 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

BMP4, ZYME, BMP2B, BMP2B1.

Quantity

$123.50

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