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Recombinant Biotin biosynthesis bifunctional protein BioAB (bioB)

Recombinant Biotin biosynthesis bifunctional protein BioAB (bioB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518751BRYYeast1mgQuote
EP518751BRYE. coli1mgQuote
BP518751BRYBaculovirus200ugQuote
MP518751BRYMammalian Cell200ugQuote

Protein Information

SpeciesBacteroides helcogenes (strain ATCC 35417 / DSM 20613 / JCM 6297 / P 36-108)
UniProt IDE6SRG2
Gene NamebioB; Locus:Bache_2094
Protein NameBiotin biosynthesis bifunctional protein BioAB Including the following 2 domains: Biotin synthase BioB
Region Expressed1-744
Expression Tag6xHis
Purity>90%
AA SequenceMTVNELKEQVLEGNFISREEAEWLAAQSDKEALYEAAHEITRTLASEEFDMCSIINAKSG RCPENCKWCAQSSHYKTKADVYDLVDKEECLRHAKYNEEQGVARFSLVTSGRKPSGKNME KLCEAARHMRRHSSIQLCASLGLLNEEEMLALHDAGITRYHCNLETAPSYFSNLCSTHTQ AEKIRTLKAARNAGMDICSGGIIGMGESMEQRIEFAFTLKDMEVQSIPINLLSPIPGTPL ERQEPLNEEEILTTIALFRFINPRAFLRFAGGRSQLSTEAVRKALHIGINSAIVGDLLTT IGSKVSEDKTLIEEAGYRFSDSQFDREHLWHPYTSTTDPLPVYKVEQAEGATITLESGQT LIEGMSSWWCAIHGYNNPVLNHAATEQIGKMSHVMFGGLTHEPAIELGKLLLPLVPPSMQ KIFYADSGSVAVEVALKMAVQYWYGKGKEKKNNFVTIRSGYHGDTWNAMSVCDPVTGMHS LFGSSLPIRYFAPQPRSRYDGDWDAGDSMELQNIIEQHHEELAALILEPIVQGAGGMWFY HPQYLREAARLCKQYGLLLIFDEIATGFGRTGKLFAWEHAGTEPDIMCIGKALTGGYMTL SAVLTTNEVADAISNHSPGVFMHGPTFMGNPLACAVACASVRLLTSPVYDWQGKVNRISM QLREELAPARQLPQVKDVRILGAIGVIEVTENVDMAWMQRRFVEEGIWVRPFGKLVYLMP PFIIEPEQLTKLTSGLIKIIKEML
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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