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Recombinant Bifunctional protein BirA (birA)

Recombinant Bifunctional protein BirA (birA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520449BRMYeast1mgQuote
EP520449BRME. coli1mgQuote
BP520449BRMBaculovirus200ugQuote
MP520449BRMMammalian Cell200ugQuote

Protein Information

SpeciesBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
UniProt IDE0U174
Gene NamebirA; Locus:BSUW23_11005
Protein NameBifunctional protein BirA Including the following 2 domains: Biotin operon repressor Biotin--[acetyl-CoA-carboxylase] synthetase
Region Expressed1-325
Expression Tag6xHis
Purity>90%
AA SequenceMRSTLRKDLIELFSQAGSEFISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYR LIKKPGKLSESEIRFGLKTEVMGQHLIYQDVISSTQKTAHELANNNAPEGTLVVADKQTA GRGRMSRVWHSQEGNGIWMSLILRPDIPLQKTPQLTLLAAVAVVQGIEAAAGIQTDIKWP NDILINGKKTVGILTEMQAEEDRVRSVIIGIGINVNQQSDDFPDELKDIATSLSQASGEK IDRAGVIQHILLCFEKRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNGTFYGKALGI DDEGVLLLETQEGIKKIYSADIELG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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