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Recombinant Bifunctional enzyme NodQ (nodQ)

Recombinant Bifunctional enzyme NodQ (nodQ) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP300336RCYYeast1mgQuote
EP300336RCYE. coli1mgQuote
BP300336RCYBaculovirus200ugQuote
MP300336RCYMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium sp. (strain N33)
UniProt IDP72339
Gene NamenodQ
Protein NameBifunctional enzyme NodQ
Region Expressed1-646
Expression Tag6xHis
Purity>90%
AA SequenceMRHIMAKSLAPTDGVRDYLAAQEKKSLLRFLTCGSVDDGKSTLIGRLLSDTKQIFEDQLA ALERDSRKHGTTGDDIDFALLVDGLEAEREQGITIDVAYRFFATPKRKFIVADTPGHEQY TRNMATGASTADLAIVLIDARQGVLRQTRRHSIIASLLGIRHIVLAVNKIDLVGFDQAVF ERITESYRQFSRDLGFQTIVPIPMSARYGDNVTSRSESMEWYSGPTLIEHLETVSVEEAV VELPFRFPVQYVNRPNLDFRGFAGTVASGSVAPGDEVVVAKSGKSSRVKRIVSYGGDLAQ AAAGQAVTLVLDDEVEISRGNMLVSPAARPQVADQFAANIVWFDEHALLPGRSYILRTET DQTSATVTDLKYRINVNDFAHEAAKSLEMNEVGICNISTRSPIAFDTFAENRTTGAFILI DRITNATVGAGMILHSLRRAENIHWQSLDVGKRVRADMKHQRPAVFWFTGLSGSGKSTIA NLFEKKLFATGRHTYILDGDNVRHGLNRDLGFTEADRVENIRRVAEVARLMADAGLIVIV SFISPFSAERRMARELMADGEFVEVFVDTPFEECARRDPKGLYARALNGEIKNFTGVDSP YEAPEKPEIHLKTLGRSAEEMVDALEHWLNERDIAQPPPADNGGSI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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