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Recombinant Bifunctional enzyme MurC/Ddl (murC/ddl)

Recombinant Bifunctional enzyme MurC/Ddl (murC/ddl) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP529510DSBYeast1mgQuote
EP529510DSBE. coli1mgQuote
BP529510DSBBaculovirus200ugQuote
MP529510DSBMammalian Cell200ugQuote

Protein Information

SpeciesChlamydia trachomatis (strain D/UW-3/Cx)
UniProt IDO84767
Gene NamemurC/ddl; Locus:CT_762
Protein NameBifunctional enzyme MurC/Ddl Including the following 2 domains: UDP-N-acetylmuramate--L-alanine ligase
Region Expressed1-803
Expression Tag6xHis
Purity>90%
AA SequenceMMKSLFYHFIGIGGIGMSALAHVLLDRGYSVSGSDLSEGKVVEKLKNKGAEFFLGNQEEH IPEGAVVVYSSSISKKNPEFLSAKSRGNRVVHRAELLAELAQDQISIFVTGSHGKTTVSS LITAILQEAKKNPSFAIGGLNQEGINGGSGSEYFVAEADESDGSIRCYTPEFSVITNIDD EHLSNFEGDRELLLASLKDFALKTQQICWYNGDCPRLRSCLQGHTFGLDSSCDLHILSYY QEGWRLYFTAKYQDVVYADIEVQLVGMHNVLNAAAAMGIALSLGIDEGAIRNAFRGFSGV QRRLQRKNSSETFLFLEDYAHHPSEISCTLRAVRTAVGQRRILAIYQPHRFSRLRECIDS FPSAFKDADEVLLTEVYSAGEEAEDISYQKLAEAISQESIVKCTHIPFHELQRHLEQSIR VHDVCVSLGAGNIVNLGEKLRDFEPQKLHLGIICGGKSCEHEISVLSAKNIAKHLSKSFY DVSYFLITREGLWESVSSLETAEDSGKSVFDPEIAQRLEKVDVVLPILHGPYGEDGAMQG FLETIGKPYTGPAIAFSAIAMNKVFTKRFMSDLGIPVVPYLPLTLAGWKQEQDKWLAHIV EAFSFPIFVKSSHLGSSIGVFEVHNVIELRDAINEAFMRDNDVFVEENRLGCKEIEVSVL GDGSGAFVVAGLHERRGSGGFIDYQEKYGLSGKSSAQIVFDTDLSKEIQEQILEAADKIY RLLLGKGSCRIDFFVDEEGNFWLSEMNPIPGMTETSPFLTSFIRKGWSYEQIVHQLVIDG LQRFNQRQRLISTSFVDQAFAIQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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