Request QuoteCatalog Number: xP526937TPRSize: 0.2-1mg

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Recombinant Bifunctional enzyme IspD/IspF (ispDF)

Recombinant Bifunctional enzyme IspD/IspF (ispDF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP526937TPRYeast1mgQuote
EP526937TPRE. coli1mgQuote
BP526937TPRBaculovirus200ugQuote
MP526937TPRMammalian Cell200ugQuote

Protein Information

SpeciesTreponema pallidum (strain Nichols)
UniProt IDO83525
Gene NameispDF; Locus:TP_0512
Protein NameBifunctional enzyme IspD/IspF Including the following 2 domains: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Region Expressed1-399
Expression Tag6xHis
Purity>90%
AA SequenceMRRGGACVQKKEYLPLTSRQPGVCLLSEILVRALEARSFFLVVVTVPAGEVAYAESQVAC DSRLSAFPSRTRPVILYVPGAHTRSASVRAGLDAMATHAPDVVLVHDGARPFVSVALIHS VLEATCRYGAAVPVIEATDTPKGVAADGSIETHLIRSRVRLAQTPQGFCYASLCAAHHRA ATDGEQYTDDSELYARYGGTVHVCAGERSNVKITYPEDLEQRASEPALTRGISVLPCTEE GALRVGLGTDMHALCAGRPLILAGIHIPSKKGAQGHSDADVLAHASIDALLGAAGLGDIG TFFPSCDGRWKDAHSCALLRHTWQLVRAACWRLVNLDAVVCLEQPALHPFREAMRASLAQ ALDTHVTRVFVKAKTAERLGPVGSGAAVTAQVVVLLKKI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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