Request QuoteCatalog Number: xP318610BOSize: 0.2-1mg

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Recombinant 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (HSD3B)

Recombinant 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (HSD3B) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP318610BOYeast1mgQuote
EP318610BOE. coli1mgQuote
BP318610BOBaculovirus200ugQuote
MP318610BOMammalian Cell200ugQuote

Protein Information

SpeciesBos taurus (Bovine)
UniProt IDP14893
Gene NameHSD3B
Protein Name3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
Region Expressed2-373
Expression Tag6xHis
Purity>90%
AA SequenceAGWSCLVTGGGGFLGQRIICLLVEEKDLQEIRVLDKVFRPEVREEFSKLQSKIKLTLLEG DILDEQCLKGACQGTSVVIHTASVIDVRNAVPRETIMNVNVKGTQLLLEACVQASVPVFI HTSTIEVAGPNSYREIIQDGREEEHHESAWSSPYPYSKKLAEKAVLGANGWALKNGGTLY TCALRPMYIYGEGSPFLSAYMHGALNNNGILTNHCKFSRVNPVYVGNVAWAHILALRALR DPKKVPNIQGQFYYISDDTPHQSYDDLNYTLSKEWGFCLDSRMSLPISLQYWLAFLLEIV SFLLSPIYKYNPCFNRHLVTLSNSVFTFSYKKAQRDLGYEPLYTWEEAKQKTKEWIGSLV KQHKETLKTKIH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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