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Recombinant Beta-galactosidase LacZ (lacZ)

Recombinant Beta-galactosidase LacZ (lacZ) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518676BQRYeast1mgQuote
EP518676BQRE. coli1mgQuote
BP518676BQRBaculovirus200ugQuote
MP518676BQRMammalian Cell200ugQuote

Protein Information

SpeciesBacillus coagulans
UniProt IDD5JGG0
Gene NamelacZ
Protein NameBeta-galactosidase LacZ
Region Expressed1-665
Expression Tag6xHis
Purity>90%
AA SequenceMLKKHEKFYYGGDYNPEQWDESVWKEDMRLMKKAGVNYVSINIFSWARLQPDEETYDFST LDKIMDMLAENGIGADLATATAAPPAWLSRKYPDSLPVDKDGSRFLPGSRQHYCPNSKDY ARLAAKLVRKIAERYKSHPALVMWHVNNEYGCHISECYCDNCKKGFQTWLKEKYGTIENL NKSWSTDFWSQRYYEWEEICLPGKTPTFANPMQQLDYKAFMDDSLLALYKMERDILKTYT PDVPVMTNLMGLHKPVDGFHWAKEMDLVTWDAYPDPFEDIPYAQFMAHDLTRSLKKQPFL LMEQAAGAVNWRAQNAVKAPGVMRLWSYEAAAHGADGIMFFQWRASQGGAEKFHSGMVPH SGDEESRNFREVVQLGNELKNLEKVTGSAYASDVAIVFDWKNWWALELDSKPSSLVTYIK QLLPFYRVLHTQNIGVDFIHPDEAMDRYKVVFAPASYRVTKTFADKVKAYVENGGYFATN FFSGIADENERVYLGGYPGAYRDILGIYVEEFAPMKKGAVHQIRTGYGDAAIRVWEEKIH LKGAEALAWFKDGYLAGSPAVTAHHCGKGKAYYIGTQPDEQYLSSLLKEILKEADVRPAL DAPRGVEVAVRKNGHEKFLFLLNHTDQVQFVDAGGTYPELIYGRTEAETVRLSPRDVKIL QVIEK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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