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Recombinant Beta-1,3-N-acetylglucosaminyltransferase radical fringe (rfng)

Recombinant Beta-1,3-N-acetylglucosaminyltransferase radical fringe (rfng) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP305256XBEYeast1mgQuote
EP305256XBEE. coli1mgQuote
BP305256XBEBaculovirus200ugQuote
MP305256XBEMammalian Cell200ugQuote

Protein Information

SpeciesXenopus laevis (African clawed frog)
UniProt IDP79949
Gene Namerfng
Protein NameBeta-1,3-N-acetylglucosaminyltransferase radical fringe
Region Expressed1-340
Expression Tag6xHis
Purity>90%
AA SequenceMKITYVGLIKVCFLVFLLLCATVLLNISWRQRDSSQSLQHCNSTCSAKYLETKLKEAHLT GRHKKWETYRLDAKPTSATGQGHQHFAKEPLQIKDLFIAVKTTKKYHGNRLNLLMQTWIS RAKEQTFIFTDWEDQELRQKAGDQMVNTNCSAVHTRQALCCKMAVEYDKFVLSDKKWFCH LDDDNYLNLHALLDLLSTFSHSTDVYVGRPSLDHPVETVDRMKGDGSGSLKFWFATGGAG FCISRGLALKMSPWASMGNFISTAEKVRLPDDCTIGYIIEGMLDVKMQHSNLFHSHLEHL QRLPTESLLKQVTLSYGGPDNKWNVVRVNGAFSLAEDPTR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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