$90.00Catalog Number: PTN641-2ugSize: 2ug

Bcl XL Human, His

B-Cell Lymphoma Extra Large Human Recombinant, His Tag

Bcl-XL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-210.
The Bcl-XL is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.

Accession

N/A

Formulation

The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Solubility

Suspend BclXL in 100ul of 0.5M Acetic acid, over night at 4oC.
Dilute 10 fold into selected buffer system.
Bcl-XL has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.

Source

Escherichia Coli.

Stability

Lyophilized Bcl-XL although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Bcl-XL should be stored at 4oC between 2-7 days and for future use below
-18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

BclXL, Bcl-X(L), Bcl-XL.

Quantity

$90.00

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