Input marker or positive control (Western Blotting).
Function study.
The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Suspend Bcl-2 in 100ul of 0.5M Acetic acid, over night at 4oC.
Dilute 10 fold into selected buffer system.
BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Bcl-2 should be stored at 4oC between 2-7 days and for future use below
-18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
