$90.00Catalog Number: PYT307-5ugSize: 5ug

BAFF Human

B-cell Activating Factor Human Recombinant

BAFF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17007 Dalton.
The BAFF is purified by proprietary chromatographic techniques.

Accession

Q9Y275

Amino acid sequence

MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL.

Biological Activity

The Human BAFF activity is determined by the dose-dependant stimulation of IL-8 production by human PBMC. The ED50 for this effect is less than 10ng/ml corresponding to a Specific Activity of 100,000IU/mg. Please note: results may vary with PBMC donors.

Formulation

Each mg of Human BAFF contains 1.6mg mannitol and 1.2mg HSA.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized BAFF in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BAFF should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.

Synonyms

BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, B-cell Activating Factor.

Quantity

$90.00

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