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Recombinant Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B

Recombinant Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP526990TPRYeast1mgQuote
EP526990TPRE. coli1mgQuote
BP526990TPRBaculovirus200ugQuote
MP526990TPRMammalian Cell200ugQuote

Protein Information

SpeciesTreponema pallidum (strain Nichols)
UniProt IDO83984
Gene NamegatB; Locus:TP_1021
Protein NameAspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B
Region Expressed1-509
Expression Tag6xHis
Purity>90%
AA SequenceMSDLQTGTVPSIAGATDDTHAAPFFYEVIIGCEIHCQLLTKTKAFCACANRSGGMPNSRV CPVCLGLPGALPVVSEEYVRLGVRAGLALGCTIQLWSAFDRKHYFYPDLPKGYQITQYDA PLCTDGAVDVQGVDMPVQRRVRIERIHLEEDAGKSLHAADAYSYIDFNRCGVPLIEIVSR PDLRSAEEAACFMQTIREILTFIEVTDGNLEEGALRCDANVNVRILYKGQEHHTPISEIK NMNSYRMVRDACTYEVQRQLQEFWQKGPASKEEMQRKRTMGWDPVEGVTLLQRTKHSLRD YRFMRDPDLPDLHLTPAYVQHLSYTVGELPAARRARFKLDLGLSAFAAQTLTGSRMLADW FEKAAHASKNARRVANWILSEVLAVVNEKNICIAELNLSPEAIAELMDAVEDQRITGKQA KDIFAQMLATGARAQDIISAQGLAQLSDEEEIATLVQTVFQEHPKALRDWQHGKTNVAAW LMGQVMKRSRGRAHPARVATLVHQALSQL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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