Request QuoteCatalog Number: xP305045MLWSize: 0.2-1mg

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Recombinant Aspartate--tRNA ligase (aspS)

Recombinant Aspartate--tRNA ligase (aspS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP305045MLWYeast1mgQuote
EP305045MLWE. coli1mgQuote
BP305045MLWBaculovirus200ugQuote
MP305045MLWMammalian Cell200ugQuote

Protein Information

SpeciesMycoplasma pneumoniae (strain ATCC 29342 / M129)
UniProt IDP75068
Gene NameaspS; Locus:MPN_046; ORFs:MP108
Protein NameAspartate--tRNA ligase
Region Expressed1-557
Expression Tag6xHis
Purity>90%
AA SequenceMNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKN KEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLR YRYLDLRRPVMQKRLALRHKVTLAVRNWLDQMGFIEVETPTLTKSTPEGARDFLVPARIR EHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEM VMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDS PNQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLP LAGTILLVAHKSKTQAWTALGAIRNELKYHLNLVKPNQYSFCWIVDFPLYEFDEKEQKWV SAHNMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGV DPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPD QVDIKDITTIWSKHPVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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