Request QuoteCatalog Number: xP303327SSQSize: 0.2-1mg

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Recombinant Aspartate--tRNA ligase (aspS)

Recombinant Aspartate--tRNA ligase (aspS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP303327SSQYeast1mgQuote
EP303327SSQE. coli1mgQuote
BP303327SSQBaculovirus200ugQuote
MP303327SSQMammalian Cell200ugQuote

Protein Information

SpeciesSynechocystis sp. (strain PCC 6803 / Kazusa)
UniProt IDP73851
Gene NameaspS; Locus:slr1720
Protein NameAspartate--tRNA ligase
Region Expressed1-599
Expression Tag6xHis
Purity>90%
AA SequenceMRTHYCGDLRTTHLGETVTLYGWVDRRRDHGGVIFLDLRDRQGIVQIASSPDQTPASYPV AEGLRNEYVVQVTGVVSKRPPESLNEKIPTGEIEIYADSIILLNGVNQQLPFVVSSHEAE QVREDVRLKYRYLDLRRARLSQNLQLRHQVVKAMRRFLEDQENFLEIETPILTRSTPEGA RDYLVPSRVNPGEWYALPQSPQLFKQLLMVAGMDRYYQIARCFRDEDLRADRQPEFTQLD MEMSFMGQEEILDLNEALICHIFKVVKNIDLPRPFPRLTYQESMAKYGNDRPDTRFGLEL VDVSDLLGNTGFKVFSAAVSSGGSIKAIRVPGGNETISNVRIKPGGDLFKEATEAGAKGI AYIRVRDNGEIDTIGAIKENLDEAQVKTLLQLTQAEAGDLLLFGAGDTATVDKSLSRLRL VLGEQLGLIDPDAINLLWITDFPMFEWNSDEKRFEALHHPFTAPHPDDLGDLKTARAQAY DLVMNGVEIGGGSLRIYQREVQEKVFATIGLSPEEAHEKFGFLLDAFEYGTPPHGGIAYG LDRLVMLLAKEDSIRDVIAFPKTQQASCLLTEAPAAVDKKQLKELSVASTYVPKVKVDD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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