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Recombinant Aspartate--tRNA ligase (aspS)

Recombinant Aspartate--tRNA ligase (aspS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP525879TPRYeast1mgQuote
EP525879TPRE. coli1mgQuote
BP525879TPRBaculovirus200ugQuote
MP525879TPRMammalian Cell200ugQuote

Protein Information

SpeciesTreponema pallidum (strain Nichols)
UniProt IDO83950
Gene NameaspS; Locus:TP_0985
Protein NameAspartate--tRNA ligase
Region Expressed1-602
Expression Tag6xHis
Purity>90%
AA SequenceMYSAHTPWYDGQVMDYPRRTIACGELRRCHVGTVVVLNGWVHRKRSHGTVSFFNMRDRSG IVQVIVSQEENASLWSTVNRIRLECCLAVEGVVRERPPSMINRALHTGEVEVHARTLYVL SENAVLPFRVDDVVHAHEDIRLKYRYLDLRSQRMQERIALRSRVALAIRQFLSMKGFIEI ETPTFICSTPEGARDFVVPSRVCPGRFYALPQSPQLYKQLLMVAGFDRYFQLARCYRDED ARGDRQPEFTQIDLEMSFVSRDDVMRVNEDMLRYVFRTSIGVELPTFFPRLTYAQALDQY GTDKPDMRFKPVLQNADFMGMLGTFTPFEEVVAQGGSIRALVLPGKARCYSRRQIEALES IARAHEAHHLFWLKATGGGLEGGIARFFAGVESEVRRRLSAQDEDLLLFVADCRHRVCCV ALGAVRSALIRDESFPEKELFSFVWIVDFPLFEWNPAENKWDPAHHMFSAPQEQYLETLE QDPGSVKGDLYDLVLNGYELASGSIRIHDTQLQKRIFKIVGLDPEEAGEKFGFLTEAFKY GAPPHGGIAHGLDRLVMLMTGSESIRDVIAFPKNTLAASPLDNCPSVLDKRQLDELHLTV HV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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