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Recombinant Asparagine--tRNA ligase (asnS)

Recombinant Asparagine--tRNA ligase (asnS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP304282MLWYeast1mgQuote
EP304282MLWE. coli1mgQuote
BP304282MLWBaculovirus200ugQuote
MP304282MLWMammalian Cell200ugQuote

Protein Information

SpeciesMycoplasma pneumoniae (strain ATCC 29342 / M129)
UniProt IDP75521
Gene NameasnS; Locus:MPN_252; ORFs:MP580
Protein NameAsparagine--tRNA ligase
Region Expressed1-455
Expression Tag6xHis
Purity>90%
AA SequenceMSATAISDLFEKPAQFKNKKIKLTGWLKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPE LFALAESISLASAVSVSGTVALTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFR NNAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHSPILTSNDCEGAGETFELKQG KEFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFAN LKDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYTKAL EILKTAQASGQANFEVQDFNFGLDLKTEHERFLCEQHFHNQPVFVINYPKDFKAFYMKQN ADGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQIDTQTLNWYLDMRKWGYFAS AGFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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