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Recombinant Arachidonate 15-lipoxygenase (loxA)

Recombinant Arachidonate 15-lipoxygenase (loxA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001621EZXYeast1mgQuote
EP001621EZXE. coli1mgQuote
BP001621EZXBaculovirus200ugQuote
MP001621EZXMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
UniProt IDQ9I4G8
Gene NameloxA; Locus:PA1169
Protein NameArachidonate 15-lipoxygenase
Region Expressed20-685
Expression Tag6xHis
Purity>90%
AA SequenceNDSIFFSPLKYLGAEQQRSIDASRSLLDNLIPPSLPQYDNLAGKLARRAVLTSKKLVYVW TENFGNVKGVPMARSVPLGELPNVDWLLKTAGVIVELIVNFVASLPASAAAQFERIATGL SGDLEAARQVHEALLEEAKNDPAAAGSLLLRFTELQTRVIAILTRVGLLVDDILKSASNL VTQRGQGDGLNRFRAVFGTLRLPEVADSFRDDEAFAYWRVAGPNPLLIRRVDALPANFPL GEEQFRRVMGADDSLLEAAASRRLYLLDYAELGKLAPSGAVDKLLTGTGFAYAPIALFAL GKDRARLLPVAIQCGQDPATHPMFVRPAESESDLYWGWQMAKTVVQVAEENYHEMFVHLA QTHLVSEAFCLATQRTLAPSHPLHVLLAPHFEGTLFINEGAARILLPSAGFIDVMFAAPI QDTQATAGGNRLGFDFYRGMLPESLKARNVDDPLALPDYPYRDDGLLVWNAIRQWAADYV AVYYASDGDVTADVELAAWVGEVIGSGKVAGFRPITGRSQLVEVLTMVIFTASAQHAAVN FPQPSMMTYAPAICAMSAAPAPDSPSGKSEADWLKMMPPTLVALEKVNIYHLLGSVYHGR LGDYRQTGFPYAPVFSDRRVTASGGPLERFQARLKEVEATIRTRNQARRRPYEYLLPSRI PASTNI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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