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Recombinant Arachidonate 15-lipoxygenase (ALOX15)

Recombinant Arachidonate 15-lipoxygenase (ALOX15) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001621PYXYeast1mgQuote
EP001621PYXE. coli1mgQuote
BP001621PYXBaculovirus200ugQuote
MP001621PYXMammalian Cell200ugQuote

Protein Information

SpeciesPongo abelii (Sumatran orangutan)
UniProt IDQ5RBE8
Gene NameALOX15
Protein NameArachidonate 15-lipoxygenase
Region Expressed2-662
Expression Tag6xHis
Purity>90%
AA SequenceGLYRIRVSTGASLYAGSNNQVQLWLVGQHGEAALGTRLWPARGKETELKVEVPEYLGPLL FVKLRKRHLLTDDAWFCNWISVQGPGAGDEVRFPCYRWVEGNGVLSLPEGTGRTVGDDPQ GLFQKHREEELEERRKLYRWGNWKDGLILNVAGAKLCDLPVDERFLEDKRVDFEVSLAKG LADLAIKDSLNVLTCWKDLDDFNRIFWCGQSKLAERVRDSWKEDALFGYQFLNGANPVVL RRSVHLPARLVFPPGMEELQAQLEKELEGGTLFEADFSLLDGIKANVILCSQQHLAAPLV MLKLQPDGKLLPMVIQLQLPSTGSPPPPLFLPTDPPMAWLLAKCWVRSSDFQLHELQSHL LRGHLMAEVIVVATMRCLPSIHPIFKLIIPHLRYTLEINVRARTGLVSDMGIFDQIMSTG GGGHVQLLKQAGAFLTYSSFCPPDDLADRGLLGVKSSFYAQDALRLWEIIYRYVEGIVSL HYKTDVAVKDDPELQTWCREITEIGLQGAQDRGFPVSLQSRDQVCHFVTMCIFTCTGQHA SVHLGQLDWYSWVPNAPCTMRLPPPTTKDATLETVMATLPNFHQASLQMSITWQLGRRQP VMVAVGQHEEEYFSGPEPKAVLKKFREELAALDKEIEIRNAKLDMPYEYLRPSIVENSVA I
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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