$148.20Catalog Number: MAB065-20ugSize: 20ug

APOA1 Antibody

Apolipoprotein A-I, Mouse Anti Human
Monoclonal Antibody

DescriptionMonoclonal antibodies provide the best target specificity and affinity. It also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Introduction

APOA1 (Apolipoprotein A-1) is a human protein with a specific role in lipid metabolism being the main protein component of HDL in the plasma. APOA1 promotes cholesterol efflux from tissues to the liver for excretion. Furthermore, APOA1 is a cofactor for LCAT, which is responsible for the formation of most plasma cholesteryl esters. In addition, APOA1 activates spermatozoa motility as part of the SPAP complex. The APOA1 gene is strongly linked with two other apolipoprotein genes on chromosome 11. Defects in the APOA1 gene are linked to HDL deficiency including Tangier disease, and with systemic non-neuropathic amyloidosis. High levels of APOA1 are linked to the manifestation of asthma and atopy.

Applications

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500~1:5000.
Recommended staring dilution is 1:1000.

Clone

PAT1E12.

Ig Subclass

Mouse IgG2a?heavy chain and?k light chain.

Immunogen

Anti-human APOA1, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human APOA1 amino acids 25-267 purified from E. coli.

Protein formulation

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Purification Method

APOA1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Storage Procedures

For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC.
Prevent freeze thaw cycles.

Synonyms

Apolipoprotein A-I, Apo-AI, ApoA-I, APOA1, MGC117399.

Type

Mouse Anti Human Monoclonal.

Quantity

$148.20

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