$148.20Catalog Number: PYT547-10ugSize: 10ug

Apo D Human

Apoliportein-D Human Recombinant

Apoliprotein-D Human Recombinant His Tag fusion protein at C-terminus (7 highlighted a.a.) produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.8 kDa.
The protein a.a.sequence corresponds to the UniProtKB/Swiss-Prot entry P05090.
The Following gene modifications were madeTrp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apoliprotein-D to enhance the protein's solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchnages which facilitate its genetic manipulation. The Apoliprotein-D is purified by proprietary chromatographic techniques.

Accession

P05090

Amino Acid Sequence

FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHHHHHH.

Formulation

Lyophilized from 1mg/ml in 4mM KH2PO4, 16mM Na2HPO4 and 115mM NaCl pH7.5.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95% as determined by SDS-PAGE.

Solubility

Add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely.

Source

Escherichia Coli.

Stability

Store lyophilized protein at -20oC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4oC for a limited period of time; it does not show any change after two weeks at 4oC.

Synonyms

Apolipoprotein D, Apo-D, ApoD.

Quantity

$148.20

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