MRGSHHHHHH GMASHMGPVQ SKSPRFASWD EMNVLAHGLL QLGQGLREHA ERTRSQLSAL ERRLSACGSA CQGTEGSTDL PLAPESRVDP EVLHSLQTQL KAQNSRIQQL FHKVAQQQRH LEKQHLRIQH LQSQFGLLDH KHLDHEVAKP ARRKRLPEMA QPVDPAHNVS RLHRLPRDCQ ELFQVGERQS GLFEIQPQGS PPFLVNCKMT SDGGWTVIQR

Western blotting, ELISA.

Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

One-step procedure using affinity Ni-NTA chromatography.

Greater than 95% as determined by SDS-PAGE.

Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited.

Escherichia Coli.

The amino acid sequence of the ANGPTL4 Human recombinant is 100% homologous to the 26-229 amino acid sequence of the Angiopoietin-like Protein-4 precursor without signal sequence.

Store lyophilized Angiopoietin-like Protein 4 Human recombinant at
-20^oC. Aliquot the ANGPTL4 after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted ANGPTL4 can be stored at
4^oC for a limited period of time; it does not show any change after two weeks at 4^oC.

ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.