MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP

Western blotting, ELISA.

ANGPTL3 Human Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

One-step procedure using affinity Ni-NTA chromatography.

Angiopoietin 5 purity is greater than 95% as determined by SDS-PAGE.

Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet of ANGPTL3 Human dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of Angiopoietin 5 is limited.

Escherichia Coli.

The amino acid sequence of the ANGPTL3 Human recombinant is 100% homologous to the 17-223 amino acid sequence of the Human Angiopoietin-like proteins-3 precursor without signal sequence.

Store lyophilized ANGPTL3 Human at -20^oC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Angiopoietin 5 can be stored at 4^oC for a limited period of time; it does not show any change after two weeks at 4^oC.

Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3.