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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP846283RKUYeast1mgQuote
EP846283RKUE. coli1mgQuote
BP846283RKUBaculovirus200ugQuote
MP846283RKUMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
UniProt IDQ92UV9
Gene NamephnW; Locus:RB0977; ORFs:SMb21537
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-383
Expression Tag6xHis
Purity>90%
AA SequenceMGEPYLLTPGPLTTAFSVKDAMLRDWGSWDGDFRGMTAELRRELLAIAGDESGQYDCVPM QGSGSFSVEAMLGSFIPRDGKVLVLMNGAYGQRIAQTLKYLGRAHVSIDKGDYMPPRGSE VAAALDADPAITHVVVVHCETSSGILNPLKEISEAVYSRGRKLLVDSMSAFGAVPAGVGD FRYEAIVSSANKCIEGVPGFGFVIARKSELEAAKDRSHSLSLDVHAQWAYMNKTGQWRFT PPTHVVAAFLEALRLHRKEGGVAGRGARYANNRDVMVAGMRQLGFETLLGDEWLSPIIVT FFSPAHPNFKFERFYELMKARGFIIYPGKLTVVDSFRIGCIGQMDSHVMQKVVAAAAESL AKMRVDTATPPALALAERARLAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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