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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP661985Yeast1mgQuote
EP661985E. coli1mgQuote
BP661985Baculovirus200ugQuote
MP661985Mammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas fluorescens (strain Pf0-1)
UniProt IDQ3K9Y3
Gene NamephnW; Locus:Pfl01_3683
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-369
Expression Tag6xHis
Purity>90%
AA SequenceMSTAAPILLTPGPLTTSARTRQAMMVDWGSWDDRFNQLTASLCEQLLAILNGADSHHCVP LQGSGTFAVEAAIGTLVPRDGKVLVLINGAYGKRLAKICEVLGRSFSTFETAEDEPTTAA DVDRLLCADSDITHVALIHCETSTGILNPLPEIAQVVEQHGKRLIIDAMSSFGALPVDAQ KVPFDALIAASGKCLEGVPGMGFVFARKESLAAAAGNSHSLAMDLFDQHSYMKKTGQWRF TPPTHVVAALHEALLQYNEEGGLPARHARYAANCQALMEEMGKLGLRSFLPAAIQAPIIA TFHAPKDPRYQFKDFYERVKAKGYILYPGKLTQVETFRVGCIGHVTPAQMREAVAAVSEV LREMEVLDI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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