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Recombinant 2-aminoethylphosphonate--pyruvate transaminase 1 (phnW1)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase 1 (phnW1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP619851BNVYeast1mgQuote
EP619851BNVE. coli1mgQuote
BP619851BNVBaculovirus200ugQuote
MP619851BNVMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia xenovorans (strain LB400)
UniProt IDQ13YI7
Gene NamephnW1; Locus:Bxeno_A2314; ORFs:Bxe_A2116
Protein Name2-aminoethylphosphonate--pyruvate transaminase 1
Region Expressed1-389
Expression Tag6xHis
Purity>90%
AA SequenceMQPRRGEPYLLTPGPLTTAFSTKEAMLRDWGSWDGDFRAMTALLRSSLLEIAGDTAGEYD CVPLQGSGSYCVEAMLGSLIPRDAHALVLANGAYGKRIVTTLGYLGRACTVLDKGDYLPP RGAEIERMLDDDPRITHVVAVHCETSSGILNPIEEIAAATAKKGRKLLIDSMSAFGAVPL DVREIACEAFVSSANKCIEGVPGFGFVIARKSALREAKGRSHSLALDVYDQWDVMNRTGQ WRFTPPTHAVAAFIEALRLFRLEGGQVGRLARYANNRDVLVAGMRELGFEPLLNARWRSP VIVTFFAPAHPSFRFETFYELMKQQGFIIYPGKLTVVDSFRIGCIGQVDEHVMRRVVEAC ANSLRTMGVGHAAPPAAALEERRTLAEAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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