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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP418119BOKYeast1mgQuote
EP418119BOKE. coli1mgQuote
BP418119BOKBaculovirus200ugQuote
MP418119BOKMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus subsp. cytotoxis (strain NVH 391-98)
UniProt IDA7GMM0
Gene NamephnW; Locus:Bcer98_1052
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-365
Expression Tag6xHis
Purity>90%
AA SequenceMKNHHYLLLTPGPLTTTKTVKEVMLYDWCTWDTEYNELVQEIRKRLVSLATKEEEYYTTV FMQGSGTFSVESVIGSVIPQEGKLLVCTNGAYGKRIVQIATTLHINVVESYTNEWEPTNL AEVEEILQKDSEITHIAVVHCETTTGIINPIADVCRLGKQYGKVTIVDAMSSFGGVEMDV ADLQIDFMISSANKCIQGVPGFGFVIAKRTELEQCKGRARSLSLDLYDQWETMELQNGKW RFTSPTHTVRAFYQALLELEEEGGVKARNTRYRNNQQVLVKRMREVGFEPLLDEAYQSPI ITSFLYPGEGFTFQQLYEELKEHGFVIYPGKISKVDTFRIGNIGDVYESDIHQLVDCISE GVVIG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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