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Recombinant 1-aminocyclopropane-1-carboxylate deaminase (acdS)

Recombinant 1-aminocyclopropane-1-carboxylate deaminase (acdS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP611209DZQYeast1mgQuote
EP611209DZQE. coli1mgQuote
BP611209DZQBaculovirus200ugQuote
MP611209DZQMammalian Cell200ugQuote

Protein Information

SpeciesCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
UniProt IDQ0K1H0
Gene NameacdS; Locus:H16_B1365
Protein Name1-aminocyclopropane-1-carboxylate deaminase
Region Expressed1-338
Expression Tag6xHis
Purity>90%
AA SequenceMNLKQFPRHSLTFGPTPIHPLSRLSESLGGKVELYAKRDDCNSGLAFGGNKTRKLEYVIP DVLAQGADTLVSIGGIQSNHTRQVAAIAAHLGLKCVLVQENWVNYSDAVYDRVGNIQMSR IMGADVRLVSEGFDIGIRPSWEEAMESVRKAGGKPYPIPAGCSEHPLGGLGYVGFAEEVR EQEAGLGFRFDYIVVCSVTGSTQAGMVVGFAADGRADRVIGIDASAKPEPTRAQILRIAR HTAELVGLGRDITEADVVLDTRYGGPEYGLPSAGTLEAIRLSARLEGMMTDPVYEGKSMQ GMIDMVRRGEFPQGSKVLFAHLGGVPALNAYSFLFKDG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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