Request QuoteCatalog Number: xP301890TNTSize: 0.2-1mg

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Recombinant Alanine--tRNA ligase (alaS)

Recombinant Alanine--tRNA ligase (alaS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP301890TNTYeast1mgQuote
EP301890TNTE. coli1mgQuote
BP301890TNTBaculovirus200ugQuote
MP301890TNTMammalian Cell200ugQuote

Protein Information

SpeciesThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
UniProt IDP74941
Gene NamealaS; Locus:TTHA1831
Protein NameAlanine--tRNA ligase
Region Expressed1-882
Expression Tag6xHis
Purity>90%
AA SequenceMRTAEIREKFLSFFEGKGHLRLPSFSLIPEDDPSLLFTSAGMAPLKPYFLGAKPIFGGRE WRRVTTCQECLRVGDIENVGRTSRHNTYFEMLGNFSFGDYFKKEAILWAWEFLTEHLKLD PGRLWVTVFEDDDEAYEIWRDLVGVPEERIGRFGEDENYWPGGAITHGPNGPSGPCSEIF YDRGPAYGTPDETGPNTGSGDRFVEIWNLVFTQYDRQGPIPGPGILKPLPQKNIDTGMGL YRVAAILQDVEDFYRTDTFFPIIQEVARMSGRPYEGKTSVSHRVIADHVRAVVAALSDGA TFSNTGRGYVIRRLLRRALRHGYLLGLSDPFLHRLAPLVAELLGDFYPEMRENLPAVEKQ IRLEEERFLETLEGGLKRLDALLSGLKPGEVLPGKEAFRLYDTYGFPLDLTVEIAAERGY GVDTEGFQKAMEEQQSRSRAAMAFEREIFKKGAQVLEELYAERGATEFLGYNALEAEAEV LALLAGDQSLLEAGPGTEVQVVLDKTPFYAEGGGQIGDFGLLEWPGGRARVETTRKTERG IFLHKARVEEGVLRVGERVRAVVDPRRRDTERNHTATHLLHAALRAVLGPHVRQAGSLVA PDRLRFDFTHPEPLKPEELERVELLVNRWIMADFPVTWRYMPLEEARKEGAMALFGEKYG EVVRVVRVEGSPLEGLESKELCGGCHVRRTGEIGAFLIRSEEAVSAGVRRIEAVTGEEAI RFARGSLNRLKALAERLEVGEAALEERLEKLLAELKEKEREVESLKARLVQAALGGGGGA SLEEKGGLRWTVAELPGLDAKALRQAADDLVARGADVALVLSGGQAVLKLSPKAQGMGLE AGALFRALAEKAGGRGGGKGALAQGGGLDPRKAREALPGLLP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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