Request QuoteCatalog Number: xP305020SSQSize: 0.2-1mg

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Recombinant Alanine--tRNA ligase (alaS)

Recombinant Alanine--tRNA ligase (alaS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP305020SSQYeast1mgQuote
EP305020SSQE. coli1mgQuote
BP305020SSQBaculovirus200ugQuote
MP305020SSQMammalian Cell200ugQuote

Protein Information

SpeciesSynechocystis sp. (strain PCC 6803 / Kazusa)
UniProt IDP74423
Gene NamealaS; Locus:sll0362
Protein NameAlanine--tRNA ligase
Region Expressed1-877
Expression Tag6xHis
Purity>90%
AA SequenceMTTTPPVLSGPEIRQQFLNFFADRQHQILPSASLVPEDPTVLLTIAGMLPFKPIFLGQKS AEFPRATTSQKCIRTNDIENVGRTARHHTFFEMLGNFSFGDYFKSQAIAWAWELSTQVFK LPAERLVVSVFEEDDEAFAIWRDEIGIPAHRIQRMGADDNFWVSGPTGPCGPCSEIYYDF HPELGDEKLDLEDDSRFIEFYNLVFMQYNRDNAGNLTPLEKKNIDTGMGLERMAQILQKV PNNYETDLIFPIIQTAANIAGIDYAQANEKTKVSLKVIGDHVRSVVHMIADGISASNLGR GYVLRRLIRRVVRHGRLLGINGEFTTKVAATAVQLAQPVYPNVLERQSLIEQELQREEAA FLKTLERGEKLLADLMADGVTEIAGADAFTLYDTFGFPLELTQEIAEEQGITVDVEGFEK AMQEQQERSKAAHETIDLTVQESLDKLANHIHPTEFLGYTDLQSSAIVKAVLVGGELVDQ AVAGQTVQIVLDQTPFYGESGGQIGDKGFLNGDNLLIRIEDVKRESGIFIHFGRVERGTV QIGTTITATIDRACRRRAQANHTATHLLQSALKRVVDEGISQAGSLVDFNRLRFDFNSPR AVTMEELQQIEDLINQWIAEAHQTEVAVMPIADAKAKGAIAMFGEKYGAEVRVIDVPGVS LELCGGTHVANTAEIGLFKIVAETGIAAGVRRIEAVAGPSVLDYLNVREAVVKELGDRLK AKPEEIPDRVHQLQQELKASQKQLEALKQELALQKSEQLLTQAQTVGEFKILVADLGTVD GESLKTAAERLQQKLGESAVVLASIPEEGKVSLVAAFSPQLVKTKQLKAGQFIGAIAKIC GGGGGGRPNLAQAGGRDASKLPEALATAKQTLLAELG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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