Request QuoteCatalog Number: xP301113MLWSize: 0.2-1mg

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Recombinant Alanine--tRNA ligase (alaS)

Recombinant Alanine--tRNA ligase (alaS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP301113MLWYeast1mgQuote
EP301113MLWE. coli1mgQuote
BP301113MLWBaculovirus200ugQuote
MP301113MLWMammalian Cell200ugQuote

Protein Information

SpeciesMycoplasma pneumoniae (strain ATCC 29342 / M129)
UniProt IDP75368
Gene NamealaS; Locus:MPN_419; ORFs:MP422
Protein NameAlanine--tRNA ligase
Region Expressed1-900
Expression Tag6xHis
Purity>90%
AA SequenceMKWTTDKVRQTWLDYFTAKGHLALPSKSLIPVNDPSLLWINSGVATLKDYFSAKKTPPSK RLANAQICLRVNDIENVGFTSRHQTLFEMLGNFSIGDYFKEEAIGFANDLLVNHYHLDPK RFYITVYQDDELTFNTWLKHGIPASRIIKCDRDRNFWDLGLGPCGPCTEIYYDRGERFDP HKVGEKLFFEDIENDRYVEVWNIVFSQFNNDGNGNYSELAQKNIDTGAGIERLVAILQDA PTNFDTDIFLKLIGIIEQHCKHKYDTNLYFKFDQKLNEAQSAFRIISDHFKAITFTIAEG VLPGPNERSYIVRRLLRRALLACKKLDLDLKFIDPMVDAIISVYGSYYQQLQGKNQVVQQ AIWKEVTAFDKTINLGLMLFEKSIAHNALQPQVAFQLYETYGFPIEMIKELVDKRQLQVD WKAVEQLMEQHRLISKQNSNTLSFEKQNEHLVNFKTASEFLYEANEITAKVIGLFDEQYQ PVQKLHNQSGYVVFDQTVLYATSGGQRYDEGYCINHSQNDQRVSFQGVFKGPNKQHFHFF LTGSFQLGDKVILVHDGKWRQLVKNNHSLEHLLHAALQNEIDPLIKQDGAFKSAQKATID FNFSRALTWAELERVEHRIRQIIQQDIQREEIFTDLEGSQKLNAIAYFEEEYSNHELLRV IRFGDFSVELCGGTHVEHTGLIENCFITDYYARGTGRWRIEIISSNETIAAYLNEQNGKL SETINSLHNTLNNIANPALNKQKTALTKQLNHFHLPQVITDLRKCQALLNELKITVNELK TEDFKWKQKQLAEKIKQELLELAKQDKAYVLASFAAVDPKLLSQVAQAVLNQHKNKLFVL LNQFNNSPSFMLLGQDVSKCIQLLKAHFELKGGGSNNFFRGSFNESVDVSKLQAILDTLQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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