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Recombinant Alanine--tRNA ligase (alaS)

Recombinant Alanine--tRNA ligase (alaS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP304900BSEYeast1mgQuote
EP304900BSEE. coli1mgQuote
BP304900BSEBaculovirus200ugQuote
MP304900BSEMammalian Cell200ugQuote

Protein Information

SpeciesBartonella bacilliformis
UniProt IDP70865
Gene NamealaS
Protein NameAlanine--tRNA ligase
Region Expressed1-886
Expression Tag6xHis
Purity>90%
AA SequenceMNSVNNIRSTFLDYFHRNGHEVLSSSPLVPRNDPTLMFTNAGMVDFKNVFTGLEKHSYNR ATTAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFSNYFKEEAIFYAWNLLTKEFCL SKDKLLVTVYHDDDVAAGLWRKISGLSEEKIIRIATNDNFWMMGDTGPCGPCSEIFYDHG DKIWGGPPGSADEDGDRFIEIWNLVFMQYEQLSKEKRIELPQPSIDTGMGLERIAAVLQG VHDNYDIDLFRTLIHASQEIIGVKATGDFFASHRVIADHLRSSAFLIADGIMPSNEGRGY VLRRIMRRAMRHAHLLGSKDLLMWRLVPVLISEMGQAYPELVRAESLISEILKLEETRFR KTLERGLGLLNEASTHLEEGDYFNGEVAFKLYDTYGFPLDLTQDALRRRGISVDVDAFDK AMKRQKAEARANWSGSGDCVTETVWFSIRDQVGATEFLGYETEKAEGIITALIRDGEVVD HIDLGQKAMIVVNQTPFYGESGGQVGDSGIISGANFIFEVHDTQKKGDNVFIHIGEIKTG QAKKHDCVELIVDSARRRKIRANHSATHLLHESLRQTLGSHVVQKGSFVSPDRLRFDFSH PKSISSEELKKIEDLANDIVLQNSKVTTRLMAIDDAIAEGAMALFGEKYGDEVRVISMGN NLEQTGSKKWWSIELCGGTHVQRTGDIGLIHIISETSVAAGVRRIEALTATAARLYLHGQ DRRVYEIAGLLKTSPADVQERVQTLLDERRKLEKELNDSRKKIALNGGSVNSQGDIQTIN GISFMGGVVSNILPKDLKALVDSGKKKIGSGVVAFISVSEDGKGSAVVGVTDDLTDTLNA VDLVRIISVTLGGQGGGGRRDMAQAGGSEGGKADEALVALKDSLKG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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