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Recombinant Alanine--tRNA ligase (alaS)

Recombinant Alanine--tRNA ligase (alaS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP528265DSBYeast1mgQuote
EP528265DSBE. coli1mgQuote
BP528265DSBBaculovirus200ugQuote
MP528265DSBMammalian Cell200ugQuote

Protein Information

SpeciesChlamydia trachomatis (strain D/UW-3/Cx)
UniProt IDO84754
Gene NamealaS; Locus:CT_749
Protein NameAlanine--tRNA ligase
Region Expressed1-875
Expression Tag6xHis
Purity>90%
AA SequenceMLSNTLRSNFLKFYANRNHTPVASSPVFPHNDPSILFTNAGMNQFKNIFLGKEQTSYTRA TTSQKCIRAGGKHNDLENVGHTSRHLTFFEMLGNFSFGDYFKQDAISFAWEVSLSIFNFD PDFIYATVHEKDDEAFALWEKYLPTDRIFRLTDKDNFWSMADTGPCGFCSELLFDRGEKF GKAASPLEDVDGERFLEYWNLVFMEFNRTSDGTLLALQKKCVDTGAGLERLVSLLAETET VFEADVLRHLISKIENLSGTTYSPTEAKGAAFRVIADHIRSLSFAIADGLLPGNTERGYV LRKILRRAVNYGKRLGFNRPFLADVVPSLVDVMGEAYPELSASVTQIQEVLTTEEEHFFK TLQRGGNLLQQVLKSSASSAKISGEDAFKLKDTYGLPIDEIALLAKDYNYAIDMDTFEKL EVEAKERSRKNTKKTKNDSDSVFQDLDPTNTSEFIGYDTLSCDTFIEGIIKYNEIASSLE EGDEGAIILRTTPFYAGKGGQIGDSGEIFCESGTFLVSHTIAPKAGLIVHLGKLSQGSLT TTMAVTAQVNQNLRKKTANNHTGCHLLHKALEMTLGEHIRQAGSYVDSQKIRLDFTHNKA LSPEDLLAIETLVNEKIRENDPVTIREVLYSDVMSSSEIKQFFGDKYGDIVRVVSAGFSH ELCGGTHAQATGDIGYFRITKEHAVATGIRRIEATTGEDAENIAREQDVDLNEIATVIQS PKDQILVKIRSVMEEKKDLAKQVADLENQLVQQQVKTLLTSCEKICDTSYLVYYLTEEEG QRIQHYANAIHKEIPTNFISLWITEKNGRYIVLSRVSDDLTKRGVQAHTLLAELLAPYGG RCGGKAISAQGSSAELPQIEFLNKTLRQWISTQLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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